Determination of total dietary fiber in foods containing resistant maltodextrin by simplified enzymatic-gravimetric method and liquid chromatography: ring test in China.
FU B, WANG L, TANG Z, ROTURIER JM AND LOOTEN P
4th International Dietary Fiber Conference, 2009 - Vienna, Austria
JEAN-MICHEL ROTURIER
Roquette Frères, Analytical Research Department, 62080 Lestrem, France
BOQIANG FU and JING WAN
National Institute of Metrology, Division of Biological, Energy and Environment Measurement, Beijing, 100013, People’s Republic of China
Abstract
An interlaboratory study was conducted in China to validate the modified AOAC Official Method 2001.03 for the determination of total dietary fiber (TDF) in foods containing resistant maltodextrin (RMD), which will be adopted as the National Standard Method of China.
The kind of buffer solution, the volume of filtrate evaporation, the volume of eluent for desalting and residual solution after evaporation, etc. were modified, which had been proved to have a acceptable accuracy and precision in the routine assay. TDF contents in 3 representative foods and 2 kinds of RMD ingredient (i.e., NUTRIOSE® 06 and NUTRIOSE® 10) were measured using the modified method in 6 eligible laboratories representing commercial, industrial, and governmental laboratories in China.
The results of the interlaboratory repeatability, interlaboratory, reproductibility, and precision of the modified method are adequate for reliable analysis of TDF in food containing RMD, as well as resistant dextrin. Compared to AOAC Official Method 2001.03, the modified method is time- and cost-saving.
Keywords: interlaboratory study – dietary fiber – resistant maltodextrin
References:
1- Official Methods of Analysis (2005) 18th, Ed., AOAC INTERNATIONAL, Gaithersburg, MD, Method 2001.03
2- Gordon, D.T., & Okuma, K. (2002) J.AOAC Int. 85, 435-444.